huh 7 5 cells (Bio-Rad)

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Bio-Rad huh 7 5 cells
Huh 7 5 Cells, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/huh+7+5+cells/Lyphochek+Urine+Metals+Control/pmc10949430-293-7-71
Average 93 stars, based on 75 article reviews

huh 7 5 cells - by Bioz Stars, 2026-11

93/100 stars

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Purification:

Article Title: Cooperation between the Hepatitis C Virus p7 and NS5B Proteins Enhances Virion Infectivity
Article Snippet: Purified linearized DNA was used as a template for in vitro transcription with a MEGAscript T7 transcription kit (Promega). .. RNA was purified to be free of DNA and transfected into Huh-7.5 cells via electroporation, as follows: 5 μg of RNA was mixed with 4 × 10 6 Huh-7.5 cells in phosphate-buffered saline (PBS) and electroporated at 250 V and 950 μF with a Gene Pulser Xcell system (Bio-Rad). ..

Transfection:

Article Title: A Disulfide-Bonded Dimer of the Core Protein of Hepatitis C Virus Is Important for Virus-Like Particle Production
Article Snippet: .. Ten micrograms of JFH1E2FL, JFH1C128A, JFH1C184A, JFH1C128/184A, JFH1PP/AA, or JFHAAA99 and J6/JFH1 or J6/JFH1AAA99 RNAs were transfected into HuH-7 and HuH-7.5 cells (1.0 107 cells) by electroporation (260 V, 0.95 F) using a Gene Pulser II system (Bio-Rad, Hercules, CA). .. Core protein expression plasmids were transfected into HuH-7 cells using Lipofectamine LTX (Invitrogen), according to the manufacturer’s protocol.

Electroporation:

Article Title: Hepatitis C Virus Infection Is Inhibited by a Noncanonical Antiviral Signaling Pathway Targeted by NS3-NS4A
Article Snippet: The in vitro -transcribed RNA was treated with DNase (Thermo Fisher), purified by phenol-chloroform extraction, and integrity verified on a denaturing gel. .. For electroporation, 1 μg of HCV replicon RNA was mixed with 4 × 10 6 Huh7 or Huh-7.5 cells in cold 1× phosphate-buffered saline (PBS) in a 4-mm cuvette and then electroporated at 960 μF and 250 V with a GenePulser XCell system (Bio-Rad). ..

Saline:

In Vitro:

Article Title: Hepatitis C virus cell culture adaptive mutations enhance cell culture propagation by multiple mechanisms but boost antiviral responses in primary human hepatocytes
Article Snippet: .. For HCVcc production, 6 × 10 6 Huh-7.5 cells in 400 μl Cytomix (120 mM KCl, 0.15 CaCl 2 , 10 mM KHP0 4 , 25 mM HEPES, 2 mM EGTA, 5 mM MgCl 2 , 2 mM ATP, 5 mM glutathione) were electroporated (270 V, 975 μF, cuvette gap width 0.4 cm) with 10 μg of in vitro transcribed HCV full-length RNA using the Gene Pulser Xcell system (BioRad). ..

other:

Article Title: Unraveling the dynamics of hepatitis C virus adaptive mutations and their impact on antiviral responses in primary human hepatocytes
Article Snippet: In vitro transcription reaction was terminated by addition of 7.5 U RQ1 Dnase (Promega) to the reaction mix and incubation for 30 min at 37°C.

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